Sperm morphology in light microscopy
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Normal sperm in the optical microscope can be roughly divided into head, body and tail of three, the head was flat oval, oval front view, side view was pear-shaped, about 4 ~ 5μm. Tail length of about 55μm, thick near the first end, a diameter of about 1μm, the end of more ...... Usually observed by optical microscopy of sperm morphology, semen must be made of smear staining. Generally use the Papanicolaou stain: Take one drop of semen (0.1 ml) in a clean glass slide, pushing into thin slices (if less sperm count in semen, semen should be first centrifugation, whichever is pushed into a uniform sheet of sediments). Set manipulation at room temperature or 37 ℃, drying oven, and then with 95% alcohol for a fixed treatment, then hematoxylin, orange and other dyes to stain EA36. By staining the front of the head of sperm stained purple red nuclear stained dark purple back of the head, body and tail to purple red. If more than unconditional special stain, can also be used generally Gram staining dye, purple stained sperm head, tail and other parts of the light red. Normal sperm in the optical microscope can be roughly divided into head, body and tail of three, the head was flat oval, oval front view, side view was pear-shaped, about 4 ~ 5μm. Tail length of about 55μm, thick near the first end, a diameter of about 1μm, smaller end of about 0.1μm, Minister of the curved tail. But also can have a normal sperm head, the physiological variation, and a big head, small head, round and pointed, naive sperm head, the head attached cap, body with appendages such as the points, old-type sperm The black head of the cytoplasm, head all the color, the head is not coloring. Abnormal sperm, including head, body and tail of the morphological variation, or the first mixing deformity. The head has a huge head deformity, head of nuclear and cytoplasmic reverse, mushroom-like head, double; body big and thick body deformity, wedge, triangle, etc.; tail deformity tail thick, chunky and forked tail and double- end; first mixing head body deformity increases, longer and head of nuclear abnormalities mixing longer and so on.