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Urgent chronic exposure of γ ray of 60 Co of M-FISH technology analysis causes
From;    Author:Stand originally

(1. Chinese CDC radiation protection and lab of radiobiology of place of nuclear safety medicine, beijing 100088; 2. Cedars-Sinai Medical Center, UCLA, los Angeles, CA 90048, USA)

In Mice Bone Marrow Cells Analyzed By Multicolor FISH Method of γ of Chromosome Aberrations Induced By Acute Or Chronic 60Co

LIU Qing-jie, CHEN De-qing, julie RK, CHEN Xiao-ning

(1. Department Of Radiation Biology, national Institute For Radiation Protection, China CDC, Beijing 100088, China; 2. Molecular Genetics Lab, Cedars-Sinai Medical Center, UCLA, CA 90048, Los Angeles, USAUSA

[summary] setting and purpose: Exploration is polychromatic and fluorescent former a cross (Multicolor Fluorescence In Situ Hybridization, m-FISH) technology analyses the difference of the distortion of small rat chromosome that illuminate of radiate of urgent chronic ionization causes. Material and method: Build with small rat 1, 2 carry bead and move with 4 chromosome filar bead particularity is artificial bacterial chromosome (Bacterial Artificial Chromosome, the M-FISH method of BAC) , the analysis gets illuminate of whole of ray of γ of urgent chronic 60Co the distortion of 1.5 chromosome of small rat marrow with 3.0 Gy, affirmatory dosage and close rate factor (Dose And Dose Rate Effect Factor, DDREF) . Result: The distortion of stability of chromosome of small rat marrow of acute exposure and blame stability distortion are identical, the small rat chromosome of chronic exposure is not stability distortion remarkable under stability distortion. Be in for index DDREF with chromosome stability distortion 1.5, 3.0 Gy is 2.2 ± respectively 0.4 with 3.1 ± 0.6. Conclusion: The distortion of chromosome of small rat marrow that M-FISH method can use at analysing ionization radiate to cause, DDREF the numerical value under the report in document.
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