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The RNA of cell of cancer of the liver that is mark with GFP turns catch a tree
From;    Author:Stand originally

[summary] purpose: Discuss GFP to observe as mark RNA of cell of cancer of the liver turns the feasibility that catchs DCs effect and tumor cell RNA turn the feasibility that catchs DCs preparation vaccine. Method: Stability of PGFPC3 of carrier of plasmid of green fluorescence albumen turns HepG2 of the cancer cell that catch liver, trizol law extracts total RNA of HepG2GFP of the cell after choosing; Blood of the week outside patient of detached liver cancer outside monocyte body revulsive DCs cell, total RNA turns catch DCs, the observation below fluorescent microscope turns catch the effect, appearance of cell drifting type detects turn catch around DCs watch change; ELISA law detects turn circumstance of change of the IL12 in catching the Qing Dynasty on around; MTT law detects effect cell is right of target cell kill and wound rate. Result: PGFPC3 stability turns the cellular HepG2GFP of GFP of attainable stable expression after HepG2 of the cancer cell that catch liver, green fluorescence is shown below fluorescent microscope; Total RNA turns caught DCs fluorescence shows green fluorescence below microscope, CD80(13.2% rises to 86.7%) , HLADR(38.9% rises to 97.9%) , CD83(0.9% rises to 97.1%) , CD86(31.2% rises to 92.5%) expression to lift apparently, clear IL12 secretes quantity Ng/L remarkable heighten on (8.1 → of 61.3 ± 287.4 ± 29.3, p < 0.05) ; Revulsive CTL can kill and wound action to the particularity since HepG2 of individual plant of cell of cancer of the liver. Conclusion: GFP can turn as tumor cell RNA catch a tree dash forward the observation mark of shape cell, tumor cell RNA turns catch DCs to be able to regard a kind of effective tumour as vaccine.

[keyword] tree dash forward shape cell; Green fluorescence albumen; RNA; Turn catch

[in plan bugle call] R739.41

0 foreword

Liver cell cancer (Hepatocellular Carcinoma, HCC) is a kind of common malignant tumor, the patient that suits operation cure holds one little share only, have higher recrudescent rate, prognosis is very poor, serious minatory mankind is healthy [1] . DCs(dendritic Cells, DCs) is the full-time sex antigen with the powerful function inside body is carried show a cell, it can be absorbed efficiently, processing antigen, give static breath T the cell the polypeptide present after processing, cause the particularity immunity that is aimed at this antigen to react [2] . In studying similarly domestic and internationally, can observe without what make clear tumor RNA turns the target that acquires DCs effect, stability of PGFPC3 of carrier of plasmid of albumen of fluorescence of our application green turns HepG2 of the cancer cell that catch liver, total RNA of HepG2GFP of the cell after extraction is chosen turns catch DCs, observation turns the element is conveyed related expression of albumen of fluorescence of green of the DCs after catching and particularity surface mark and function, detect clear cell factor is secreted on its, discuss its to regard tumor as vaccinal feasibility.
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